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Issue 19, 2017
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Gold nanoparticle aggregation enables colorimetric sensing assays for enzymatic decarboxylation

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Abstract

Monitoring enzymatic processes is of fundamental importance for the understanding of the associated biological phenomena. In this work, we show that amino acid decarboxylase activity can be readily monitored by a colorimetric assay with gold nanoparticles (AuNPs), which undergo selective product-induced aggregation. Once the carboxylate group is eliminated, the resulting diamine products trigger AuNP aggregation, leading to a significant color change. Furthermore, this new method provides a rapid way for potential inhibitor screening.

Graphical abstract: Gold nanoparticle aggregation enables colorimetric sensing assays for enzymatic decarboxylation

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Publication details

The article was received on 09 Mar 2017, accepted on 18 Apr 2017 and first published on 19 Apr 2017


Article type: Communication
DOI: 10.1039/C7AY00642J
Citation: Anal. Methods, 2017,9, 2784-2787
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    Gold nanoparticle aggregation enables colorimetric sensing assays for enzymatic decarboxylation

    M. Nilam, A. Hennig, W. M. Nau and K. I. Assaf, Anal. Methods, 2017, 9, 2784
    DOI: 10.1039/C7AY00642J

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