Jump to main content
Jump to site search


Gold Nanoparticle Aggregation Enables Colorimetric Sensing Assays for Enzymatic Decarboxylation

Abstract

Monitoring enzymatic processes is of fundamental importance for the understanding of the associated biological phenomena. In this work, we show that amino acid decarboxylase activity can be readily monitored by a colorimetric assay with gold nanoparticles (AuNPs), which undergo selective product-induced AuNPs aggregation. Once the carboxylate group is eliminated, the resulting diamine products trigger AuNPs aggregation, leading to a significant color change. Furthermore, the new method provides a rapid way for potential inhibitor screening.

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 09 Mar 2017, accepted on 18 Apr 2017 and first published on 19 Apr 2017


Article type: Communication
DOI: 10.1039/C7AY00642J
Citation: Anal. Methods, 2017, Accepted Manuscript
  •   Request permissions

    Gold Nanoparticle Aggregation Enables Colorimetric Sensing Assays for Enzymatic Decarboxylation

    M. Nilam, A. Hennig, W. M. Nau and K. I. Assaf, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00642J

Search articles by author