Gold Nanoparticle Aggregation Enables Colorimetric Sensing Assays for Enzymatic Decarboxylation
Monitoring enzymatic processes is of fundamental importance for the understanding of the associated biological phenomena. In this work, we show that amino acid decarboxylase activity can be readily monitored by a colorimetric assay with gold nanoparticles (AuNPs), which undergo selective product-induced AuNPs aggregation. Once the carboxylate group is eliminated, the resulting diamine products trigger AuNPs aggregation, leading to a significant color change. Furthermore, the new method provides a rapid way for potential inhibitor screening.