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Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

Abstract

Almost all the existing assay methods for alkaline phosphatase (ALP) require expensive analytical instruments and well-trained users to achieve quantitative results. This work initially describes a quantitative ALP assay that integrates low-cost microfluidic paper-based analytical devices (μPADs) with the instrument-free length-measuring readout. The detection motif of length measurement is based on the starch-mediated wettability change of the μPAD from hydrophilic to hydrophobic. Under optimal conditions, this new method is quantitatively sensitive to ALP levels in buffer samples ranging from ~0.075 to 5 U/mL, with a visual detection limit of 0.075 U/mL. The satisfactory recovery results of assaying several human serum samples additionally prove its practicability. The proposed method needs only a ubiquitous cheap ruler to realize quantitative analysis, thus making it a simple, cost-efficient, and promising alternative tool for potential broad applications in diagnosis of ALP-relevant diseases or assessment of ALP functions in biological systems especially in resource-poor settings.

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Publication details

The article was received on 06 Mar 2017, accepted on 05 May 2017 and first published on 08 May 2017


Article type: Technical Note
DOI: 10.1039/C7AY00599G
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

    L. Zhang, J. Nie, H. Wang, J. Yang, B. Wang, Y. Zhang and J. Li, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00599G

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