Jump to main content
Jump to site search

Issue 22, 2017
Previous Article Next Article

Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

Author affiliations

Abstract

Almost all the existing assay methods for alkaline phosphatase (ALP) require expensive analytical instruments and well-trained users to achieve quantitative results. This work initially describes a quantitative ALP assay that integrates low-cost microfluidic paper-based analytical devices (μPADs) with an instrument-free length-measuring readout. The detection motif of length measurement is based on the starch-mediated wettability change of the μPAD from hydrophilic to hydrophobic. Under optimal conditions, this new method is quantitatively sensitive to ALP levels in buffer samples ranging from ∼0.075 to 5 U mL−1, with a visual detection limit of 0.075 U mL−1. The satisfactory recovery results of assaying several human serum samples additionally prove its practicability. The proposed method needs only a ubiquitous cheap ruler to realize quantitative analysis, thus making it a simple, cost-efficient, and promising alternative tool for potential broad applications in the diagnosis of ALP-relevant diseases or assessment of ALP functions in biological systems especially in resource-poor settings.

Graphical abstract: Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Mar 2017, accepted on 05 May 2017 and first published on 08 May 2017


Article type: Technical Note
DOI: 10.1039/C7AY00599G
Citation: Anal. Methods, 2017,9, 3375-3379
  •   Request permissions

    Instrument-free quantitative detection of alkaline phosphatase using paper-based devices

    L. Zhang, J. Nie, H. Wang, J. Yang, B. Wang, Y. Zhang and J. Li, Anal. Methods, 2017, 9, 3375
    DOI: 10.1039/C7AY00599G

Search articles by author

Spotlight

Advertisements