Development of an analytical method for the determination of n-nitrosamines in tobacco by GC-NCD after solid phase extraction.
In this paper an analytical method was developed for the determination of some tobacco specific n-nitrosamines (TSNA) in cigarettes, and applied to cigarettes purchased in the city of Rio de Janeiro / RJ (Brazil). The analyte detection is based on the gas chromatography technique with chemiluminescence detector (GC-NCD), while the extraction is based on two stages, the first is a cigarette leaching step, and another is a solid phase extraction of TSNA from this leaching. The method presented accuracy of 92 + 18%, the precision was calculated using the coefficient of variation of a standard TSNA mix solution measured on 3 successive days and was in the range from 4.7 to 6.8%. The working range of all TSNA was 50-700 ng mL-1, allowing the method to have LOQ values from 198.3 to 623.7.1 ng cig-1. The method developed allowed determination of NNN, NAT, NNK and NAB in commercial cigarette samples.