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Issue 15, 2017
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Bioluminescent detection of tick-borne encephalitis virus in native ticks

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Abstract

A one-step bioluminescent immunoassay for tick-borne encephalitis virus (TBEV) in natural ticks based on the hybrid protein 14D5a-Rm7 was developed. Recombinant Ca2+-dependent coelenterazine-binding protein was shown to be a more convenient substrate form for the Rm7 domain than coelenterazine. Over 600 samples of natural ticks were analyzed and shown to have essential differences in the discrimination factor D for TBEV-positive (2.77 ± 0.81) and TBEV-negative (1.15 ± 0.28) samples.

Graphical abstract: Bioluminescent detection of tick-borne encephalitis virus in native ticks

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Publication details

The article was received on 28 Feb 2017, accepted on 21 Mar 2017 and first published on 22 Mar 2017


Article type: Paper
DOI: 10.1039/C7AY00535K
Citation: Anal. Methods, 2017,9, 2252-2255
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    Bioluminescent detection of tick-borne encephalitis virus in native ticks

    A. N. Kudryavtsev, L. P. Burakova and L. A. Frank, Anal. Methods, 2017, 9, 2252
    DOI: 10.1039/C7AY00535K

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