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New insights into supercritical fluid chromatography for chiral separations

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Abstract

Bioanalysis of stereoisomers represents a great challenge, as chromatographic separation is not feasible using conventional reversed or normal-phase chromatography. Supercritical fluid chromatography in combination with chiral stationary phases has proven to be a great tool for chiral resolution, alleviating some of the challenges associated with bioanalysis of stereoisomers. This technical note describes an alternative to the traditional SFC instrument configuration without the use of a back pressure regulator (BPR) and makeup pump; thereby simplifying its usage and improving the robustness and quality of measurements. This alternative configuration has been successfully employed in our laboratory for multiple chiral applications; in particular for the separation of cis/trans geometric stereoisomers. Four case studies are presented – one of which was validated demonstrating the robustness of the technology for bioanalytical applications.

Graphical abstract: New insights into supercritical fluid chromatography for chiral separations

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Publication details

The article was received on 20 Feb 2017, accepted on 26 Mar 2017 and first published on 28 Mar 2017


Article type: Technical Note
DOI: 10.1039/C7AY00452D
Citation: Anal. Methods, 2017, Advance Article
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    New insights into supercritical fluid chromatography for chiral separations

    H. Licea-Perez and C. A. Evans, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY00452D

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