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Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

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Abstract

Li+-Doped NaYF4:Yb,Er upconversion luminescent nanoparticles (UCLNPs) were synthesized and modified with poly(acrylic acid) (PAA). The luminescence of the carboxyl-functionalized UCLNPs at 664 nm was quenched in the presence of cupric ions (Cu2+) due to the coordination of Cu2+ with the carboxyl group of PAA. When pyrophosphate ions (PPi) were added, the luminescence of the UCLNPs was restored due to the competitive binding of PPi with Cu2+. However, pyrophosphatase (PPase) could promote the hydrolysis of PPi, thus releasing Cu2+, leading to requenching of the luminescence. Under optimal conditions, the luminescence response was virtually proportional to the activity of the PPase between 0.500 and 50.0 mU mL−1, and the limit of detection was 0.441 mU mL−1. Based on the competition assay approach, a new highly sensitive method in the red region for real-time detection of PPase activity was developed.

Graphical abstract: Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

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Publication details

The article was received on 20 Feb 2017, accepted on 04 May 2017 and first published on 08 May 2017


Article type: Paper
DOI: 10.1039/C7AY00444C
Citation: Anal. Methods, 2017, Advance Article
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    Real-time assay of inorganic pyrophosphatase activity in the red region based on Li+-doped NaYF4:Yb,Er upconversion luminescent nanoparticles

    L. Wang, Y. Ji, Y. He, L. Wang and H. Chen, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY00444C

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