Jump to main content
Jump to site search


Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Author affiliations

Abstract

Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in the Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important, given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of ART on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 μM and with 0.329 mA mM−1 sensitivity. The calculated apparent Michaelis–Menten constant Km for hemoglobin anchored on carbon nanofibers towards catalysis of ART was 0.093 mM. The analysis of free hemoglobin reacted with ART monitored using UV-visible spectroscopy and liquid chromatography indicated formation of adducts.

Graphical abstract: Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 17 Feb 2017, accepted on 20 Apr 2017 and first published on 20 Apr 2017


Article type: Paper
DOI: 10.1039/C7AY00431A
Citation: Anal. Methods, 2017, Advance Article
  •   Request permissions

    Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

    Z. Mazzochette, E. Newton and A. Mugweru, Anal. Methods, 2017, Advance Article , DOI: 10.1039/C7AY00431A

Search articles by author