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Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

Abstract

Abstract Artemisinin (ART) is a sesquiterpene lactone and a popular malaria drug used in Asian subcontinent and some parts of Africa. Understanding the mechanism of action of this drug is important given recent reports of emergence of resistance of malaria to ARTs. We have investigated biocatalytic reduction of artemisinin on hemoglobin anchored on carbon nanofibers using electrochemistry. We have also investigated the drug interaction with hemoglobin in free solution using liquid chromatography and UV-visible spectroscopy. Using electrochemistry, linear relationships between the catalytic peak current and the ART concentration was observed. The linear range was from 0 to 200 µM and with 0.329 mA/mM sensitivity. The calculated apparent Michaelis-Menten constant Km for hemoglobin anchored on carbon nanofiber towards catalysis of ART was 0.093 mM. Analysis of free hemoglobin reacted with artemisinin monitored using UV-Visible spectroscopy and liquid chromatography indicated formation of adducts.

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Publication details

The article was received on 17 Feb 2017, accepted on 20 Apr 2017 and first published on 20 Apr 2017


Article type: Paper
DOI: 10.1039/C7AY00431A
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Electrochemical catalysis of artemisinin on hemoglobin functionalized carbon nanofibers

    Z. Mazzochette, N. Ebenezer and A. Mugweru, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00431A

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