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Spectroscopy Study of the Interaction between Endocrine Disruptor 4-OH-2, 2’, 3, 4’-BDE and Human Serum Albumin

Abstract

Hydroxylated polybrominated diphenyl ethers (OH-PBDEs), as one of the derivative of polybrominated diphenyl ethers (PBDEs), have received extensive concern recently. In this paper, the interaction of OH-PBDEs and human serum albumin (HSA) was investigated using 4-OH-2, 2’, 3, 4’-tetrabromodiphenyl ether (4-OH-BDE-42) as a representative. The binding constant, the number of binding sites and binding site were investigated using fluorescence spectroscopy. The predominant forces of the interaction were Van der Waals and hydrogen bonds which was made clear by the calculation of thermodynamic parameters. The alterations of HSA protein secondary structure in presence of 4-OH-BDE-42 were confirmed by UV-Vis and CD spectroscopy. The distance between HSA to 4-OH-BDE-42 was studied by Förster’s non-radioactive energy transfer theory. Equilibrium dialysis experiment was performed and indicated that 4-OH-BDE-42 severely affects the physiological function of HSA in transportation of T3, Trp and VB2. This paper had made deep investigation and detailed interpretation to the interaction of 4-OH-BDE-42 and HSA.

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Publication details

The article was received on 17 Feb 2017, accepted on 05 May 2017 and first published on 08 May 2017


Article type: Paper
DOI: 10.1039/C7AY00419B
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Spectroscopy Study of the Interaction between Endocrine Disruptor 4-OH-2, 2’, 3, 4’-BDE and Human Serum Albumin

    Z. Chen, S. Hu, X. He, L. Guo, B. Qiu, Z. Lin, G. Chen and Z. Cai, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00419B

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