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Direct and interference-free determination of thirteen phenolic compounds in red wines using chemometrics-assisted HPLC-DAD strategy for the authentication of vintage year

Abstract

In the present work, a novel chemometrics-assisted analytical strategy that combines three-way high performance liquid chromatography-diode array detection (HPLC-DAD) data with second-ord- er calibration method based on alternating trilinear decomposition (ATLD) algorithm was developed for direct, accurate and simultaneous determination of thirteen phenolic compounds in complex red wine samples without intricate clean-up step. All analytes were fast eluted out (7.5 min) under a sim-ple gradient LC-separation and then detected in a multi-channel UV window. With the aid of promi-nent "second-order advantage" of ATLD algorithm, four common HPLC problems, i.e. solvent peak, peak overlaps, unknown interferences and baseline drifts could be mathematically calibrated, making "pure signals" of analytes could be extracted out from heavily interferent¬ial but information-rich HPLC-DAD profiles. The new strategy could avoid the loss of analytes of interest to significantly improve analytical accuracy. Validation parameters, i.e. recovery (97.7-104%), precision (RSD < 7.1%), matrix effect, limits of detection (LODs, 0.02-0.27μg mL-1) and limits of quantitation (LOQs, 0.06-0.82μg mL-1) of thirteen analytes, were surveyed and further confirmed by LC-MS/MS method. Based on the indexes of phenolic compositions in wines, pattern recognition methods, i.e. principal component analysis and linear discriminant analysis (PCA-LDA) were applied for distinguishing wines of different storage years, the discriminant accuracies were higher than 90%, which proved that this chemometrics-assisted HPLC-DAD strategy was an excellent method for direct and accurate determination of phenolic compositions in complex wine samples as well as the authentica-tion of vintage year.

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Publication details

The article was received on 16 Feb 2017, accepted on 26 Apr 2017 and first published on 27 Apr 2017


Article type: Paper
DOI: 10.1039/C7AY00415J
Citation: Anal. Methods, 2017, Accepted Manuscript
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    Direct and interference-free determination of thirteen phenolic compounds in red wines using chemometrics-assisted HPLC-DAD strategy for the authentication of vintage year

    Z. Liu, H. Wu, L. Xie, Y. Hu, H. Fang, X. Sun, T. Wang, R. Xiao and R. Yu, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY00415J

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