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Issue 23, 2017
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Integrating qualitative and quantitative assessments of Yougui pill, an effective traditional Chinese medicine, by HPLC-LTQ-Orbitrap-MSn and UPLC-QqQ-MS/MS

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Abstract

Yougui pill (YGP) is a well-known Chinese medicine formula with definite clinical effects on kidney-yang deficiencies such as infertility. However, Aconitum carmichaeli var. carmichaeli, one of the herbs contained in YGP, shows several side effects. Therefore, it is important to carry out strict and systematic quality evaluation on YGP to ensure its effects and safety. In the present study, qualitative and quantitative analyses were performed on YGP. First, an HPLC-LTQ-Orbitrap-MSn method was applied to validate the chemical profile of YGP. Eighty-three compounds were identified, including iridoids, flavones and alkaloids. Second, an UPLC-QqQ-MS/MS method was used for the simultaneous quantitation of 16 targeted compounds in YGP. The method showed a good linear correlation (R > 0.995) over a wide range and a high sensitivity, as established by LOD (0.04–0.40 ng mL−1) and LOQ (0.10–1.00 ng mL−1). The RSD values of intra- and inter-day precisions were less than 10%. The RSD values of repeatability and stability were less than 5%, and recovery assays were between 85.8% and 96.2%. The cluster analysis indicated that the qualities of YGP from two manufacturers were very different. The present integrated qualitative and quantitative assessment of YGP provides a good example of quality evaluation strategy to assess Chinese medicine formulas.

Graphical abstract: Integrating qualitative and quantitative assessments of Yougui pill, an effective traditional Chinese medicine, by HPLC-LTQ-Orbitrap-MSn and UPLC-QqQ-MS/MS

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Publication details

The article was received on 27 Jan 2017, accepted on 16 May 2017 and first published on 16 May 2017


Article type: Paper
DOI: 10.1039/C7AY00259A
Citation: Anal. Methods, 2017,9, 3485-3496
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    Integrating qualitative and quantitative assessments of Yougui pill, an effective traditional Chinese medicine, by HPLC-LTQ-Orbitrap-MSn and UPLC-QqQ-MS/MS

    H. Liu, F. Qiu, B. Bian, X. Yang, H. Zou, H. Zhao, Z. Chen, Q. Wang, H. Zhao and L. Wang, Anal. Methods, 2017, 9, 3485
    DOI: 10.1039/C7AY00259A

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