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Issue 10, 2017
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Detection of cortisol in serum using quantitative resonance Raman spectroscopy

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Abstract

Measurement of cortisol in serum is used commonly as an indicator of stress and disease. Conventional analytical techniques have limited utility given that they remain largely laboratory based, they do not directly measure the deemed biologically active free cortisol, and there is no robust correlation between the free cortisol measurements within serum and saliva. It would therefore be desirable to measure both the free and total cortisol readily within the same matrix in a portable device in the field or at the bedside. This paper demonstrates the utility of a portable Raman approach to measure both the biologically active free cortisol as well as total cortisol in human serum, compared to a laboratory-based chemiluminescence analysis technique. This alternative portable Raman method produced results that were consistent with results obtained from previous methods, which has the potential for further miniaturisation for point of test applications.

Graphical abstract: Detection of cortisol in serum using quantitative resonance Raman spectroscopy

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Publication details

The article was received on 07 Dec 2016, accepted on 12 Feb 2017 and first published on 13 Feb 2017


Article type: Paper
DOI: 10.1039/C6AY03296F
Citation: Anal. Methods, 2017,9, 1589-1594
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    Detection of cortisol in serum using quantitative resonance Raman spectroscopy

    K. Gracie, S. Pang, G. M. Jones, K. Faulds, J. Braybrook and D. Graham, Anal. Methods, 2017, 9, 1589
    DOI: 10.1039/C6AY03296F

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