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Bulk double emulsification for flow cytometric analysis of microfluidic droplets

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Abstract

Droplet microfluidics is valuable for applications in chemistry and biology, but generates massive numbers of droplets that must be analyzed and sorted. Here, we describe a simple approach to bulk double emulsify microfluidic emulsions for analysis and sorting with commercial flow cytometers. We illustrate the method by using it to identify droplets based on nucleic acid content. Though simple, our method provides a general approach for analyzing and sorting microfluidic droplets without custom microfluidic double emulsifiers or sorters.

Graphical abstract: Bulk double emulsification for flow cytometric analysis of microfluidic droplets

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Publication details

The article was received on 13 Oct 2017, accepted on 16 Oct 2017 and first published on 13 Nov 2017


Article type: Communication
DOI: 10.1039/C7AN01695F
Citation: Analyst, 2017, Advance Article
  • Open access: Creative Commons BY-NC license
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    Bulk double emulsification for flow cytometric analysis of microfluidic droplets

    D. J. Sukovich, S. C. Kim, N. Ahmed and A. R. Abate, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN01695F

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