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Separation of proteins from complex bio-matrix samples by using a double-functionalized polymer monolithic column

Abstract

A double-functionalized polymer monolithic column was fabricated within the confines of a stainless steel column (50 mm × 4.6 mm i.d.) via a facile and fast method using iron porphyrin, ionic liquid (1-allyl-3-methylimidazolium chloride) and 1,10-decanediol dimethacrylate as tri-monomers; ethylene dimethacrylate as crosslinker; polyethylene glycol 400 and N, N-dimethylformamide as co-porogens; benzoyl peroxide and N,N-dimethyl aniline as redox initiation system. Results obtained from scanning electron microscope, nitrogen adsorption-desorption instrument, and mercury intrusion porosimeter confirmed the uniform pore structure and macro-pore size distribution of the resulting monoliths, respectively. The home-made monolith was furtherly characterized by elementary analyzer to investigate the element composition of Fe supplied by iron porphyrin, confirming the synthetic process. The resulting optimized monolithic column was used as stationary phase of high performance liquid chromatography for separating proteins, such as mixture of standard proteins, egg white, and human plasma, which exhibited good selectivity and high performance. It is worth implying that the homemade double-functionalized polymer monolithic column has shown excellent selectivity for fractionation separation of human plasma proteins, and it is a promising separation method for complex bio-samples in proteomic research.

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Supplementary files

Publication details

The article was received on 08 Sep 2017, accepted on 11 Nov 2017 and first published on 14 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01491K
Citation: Analyst, 2017, Accepted Manuscript
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    Separation of proteins from complex bio-matrix samples by using a double-functionalized polymer monolithic column

    D. Zhang, D. Lan, X. Pang, B. Cui, L. Bai, H. Liu and H. Yan, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01491K

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