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Aptamer and functionalized nanoparticles-based strip biosensor for on-site detection of kanamycin in food samples

Abstract

A lateral flow strip biosensor for fast, sensitive, low-cost and on-site detection of kanamycin was developed by using kanamycin-specific aptamer-modified gold nanoparticles (AuNPs-apt) as probe and oligonucleotide DNA1-modified silver nanoparticles (AgNPs-DNA1) as signal amplification element. Through the complementary sequences of DNA1 and the aptamer, AgNPs-DNA1-apt-AuNPs complex can be formed and further captured on the test zone of the strip, where a capture probe DNA2 complementary to 3’-terminal of DNA1 was immobilized. The presence of kanamycin can competitively bind to the aptamer, then inhibit the formation of the complex and the accumulation of AuNPs on the test zone. AuNPs-apt can finally be captured on the control zone via the specific binding between biotin and streptavidin. The assay avoids the multiple incubation and washing steps, and can be completed within 10 min. By observing the color change of the test zone, a qualitative detection for kanamycin can be achieved by naked eyes, with the visual limit of 35 nM. Meanwhile, a linear detection range of 1-30 nM with a low detection limit of 0.0778 nM for quantitative analysis can be achieved by using a scanning reader. The lateral flow strip biosensor exhibited high specificity and stability. Moreover, it was applied to detect kanamycin in various food samples, indicating its great potential in field testing.

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Publication details

The article was received on 06 Sep 2017, accepted on 08 Nov 2017 and first published on 08 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01476G
Citation: Analyst, 2017, Accepted Manuscript
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    Aptamer and functionalized nanoparticles-based strip biosensor for on-site detection of kanamycin in food samples

    J. Liu, J. Zeng, Y. Tian and N. Zhou, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01476G

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