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Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling

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Abstract

One of the promising approaches to address the challenge of detecting dilute nucleic acid analytes is exonuclease III-aided target recycling. In this strategy, the target DNA self-assembles with the reactant DNA probes and displays itself as a reactant and product at the same time. This provides an autonomous mechanism to release and reuse the analyte from each round of reactions for repetitive cycles, which amplifies the signal without amplifying the analyte itself. However, for very low amounts of the analyte, it takes a considerably long time before a detectable signal is generated. Thus, in this paper, we report a kinetically-enhanced target recycling strategy by designing two more target recycling sub-reactions that are triggered by the byproducts of the first reaction involving the target analyte. In this manner, concentrations of up to 0.5 pM of target DNA can be detected in 15 minutes.

Graphical abstract: Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling

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Publication details

The article was received on 28 Aug 2017, accepted on 05 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01423F
Citation: Analyst, 2017, Advance Article
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    Kinetically-enhanced DNA detection via multiple-pass exonuclease III-aided target recycling

    H. L. Lee Yu, Y. Zhang and I. Hsing, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN01423F

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