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Issue 23, 2017
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Quantification of a bacterial secondary metabolite by SERS combined with SLM extraction for bioprocess monitoring

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Abstract

During the last few decades, great advances have been reached in high-throughput design and building of genetically engineered microbial strains, leading to a need for fast and reliable screening methods. We developed and optimized a microfluidic supported liquid membrane (SLM) extraction device and combined it with surface enhanced Raman scattering (SERS) sensing for the screening of a biological process, namely for the quantification of a bacterial secondary metabolite, p-coumaric acid (pHCA), produced by Escherichia coli. The microfluidic device proved to be robust and reusable, enabling efficient removal of interfering compounds from the real samples, reaching more than 13-fold up-concentration of the donor at 10 μL min−1 flow rate. With this method, we quantified pHCA directly from the bacterial supernatant, distinguishing between various culture conditions based on the pHCA production yield. The obtained data showed good correlation with HPLC analysis.

Graphical abstract: Quantification of a bacterial secondary metabolite by SERS combined with SLM extraction for bioprocess monitoring

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Publication details

The article was received on 22 Aug 2017, accepted on 17 Oct 2017 and first published on 17 Oct 2017


Article type: Paper
DOI: 10.1039/C7AN01393K
Citation: Analyst, 2017,142, 4553-4559
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    Quantification of a bacterial secondary metabolite by SERS combined with SLM extraction for bioprocess monitoring

    L. Morelli, S. Z. Andreasen, C. B. Jendresen, A. T. Nielsen, J. Emnéus, K. Zór and A. Boisen, Analyst, 2017, 142, 4553
    DOI: 10.1039/C7AN01393K

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