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Issue 20, 2017
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A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T

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Abstract

A glass fibre membrane platform that allows quantification of circulating cTnT with a LoD of 0.87 pg mL−1 is described. The proposed platform uses a glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1–120 pg mL−1 at room temperature in 30 min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB-cTnT-cAB-DNA) containing complementary oligonucleotides. There were no interferences from human cTnI, cTnC, skTnT, biotin, and hemoglobin (each 1 μg mL−1). The linearity in the serial dilution test of plasma samples indicates that this platform is highly applicable for regular health check-up to assess the risk of AMI and HF.

Graphical abstract: A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T

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Publication details

The article was received on 22 Aug 2017, accepted on 04 Sep 2017 and first published on 04 Sep 2017


Article type: Paper
DOI: 10.1039/C7AN01389B
Citation: Analyst, 2017,142, 3816-3821
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    A glass fibre membrane platform for ultra-sensitive detection of cardiac troponin T

    K. Song, S. B. Nimse, M. D. Sonawane, Y. Lin, Z. Zhou and T. Kim, Analyst, 2017, 142, 3816
    DOI: 10.1039/C7AN01389B

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