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Glass Fibre Membrane Platform for Ultra-sensitive Detection of Cardiac Troponin T

Abstract

A glass fibre membrane platform that allows quantification of circulating cTnT with LoD of 0.87pg/mL is described. The proposed platform uses glass fibre membrane, DNA-guided detection method, and antibody-conjugated fluorescent beads for the quantification of cTnT in the analytical detection range of 1-120pg/mL at room temperature in 30min. Glass fibre membranes were chemically modified to immobilize the oligonucleotide probes that catch a biomolecular complex (FB-dAB- cTnT- cAB-DNA) containing complementary oligonucleotides. There were no interferences from human cTnI, cTnC, skTnT, biotin, and hemoglobin (each 1µg/mL). The linearity in the serial dilution test of plasma samples indicates that this platform is highly applicable for regular health check-up to assess the risk of AMI and HF.

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Publication details

The article was accepted on 04 Sep 2017 and first published on 04 Sep 2017


Article type: Paper
DOI: 10.1039/C7AN01389B
Citation: Analyst, 2017, Accepted Manuscript
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    Glass Fibre Membrane Platform for Ultra-sensitive Detection of Cardiac Troponin T

    T. Kim, K. Song, S. B. Nimse, M. D. Sonawane, Y. Lin and Z. Zhou, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01389B

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