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Issue 21, 2017
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Gold–platinum bimetallic nanoclusters with enhanced peroxidase-like activity and their integrated agarose hydrogel-based sensing platform for the colorimetric analysis of glucose levels in serum

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Abstract

This work presents a novel and facile strategy for the fabrication of gold–platinum bimetallic nanoclusters (Au–PtNCs) with adjustable Au/Pt molar ratios by a one-pot synthetic route. It was unexpectedly found that the prepared Au–PtNCs with an optimal Au/Pt molar ratio (1 : 1) could exhibit greatly enhanced peroxidase-like catalytic activity and chemical stability toward harsh conditions due to the synergistic effect of the two atoms, in contrast with pure AuNCs. These prominent advantages render Au–PtNCs capable of sensitive and selective colorimetric detection of glucose by means of a NCs–glucose oxidase (GOx) cascade-catalyzed system using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate. This assay can be used not only for visual detection of glucose by the naked eye but for reliable and convenient quantification in the range from 5 to 55 μM with a detection limit of 2.4 μM. Importantly, to widen the application of point-of-care testing (POCT) of glucose to biomedical diagnosis, an integrated agarose hydrogel-based sensing platform comprising NCs, GOx and TMB was rationally designed. It was demonstrated that this sensing platform could serve as a reagentless and instrument-free platform for direct visualization of glucose with different levels in human serum, as the results were in good accordance with those obtained from a free NC-involved detection system as well as from a commercial blood glucometer.

Graphical abstract: Gold–platinum bimetallic nanoclusters with enhanced peroxidase-like activity and their integrated agarose hydrogel-based sensing platform for the colorimetric analysis of glucose levels in serum

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Publication details

The article was received on 12 Aug 2017, accepted on 11 Sep 2017 and first published on 14 Sep 2017


Article type: Paper
DOI: 10.1039/C7AN01343D
Citation: Analyst, 2017,142, 4106-4115
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    Gold–platinum bimetallic nanoclusters with enhanced peroxidase-like activity and their integrated agarose hydrogel-based sensing platform for the colorimetric analysis of glucose levels in serum

    J. Feng, P. Huang and F. Wu, Analyst, 2017, 142, 4106
    DOI: 10.1039/C7AN01343D

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