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Towards Single Egg Toxicity Screening Using Microcoil NMR

Abstract

Planar microcoils ranging from 20-1000 μm I.D. (130-1130 μm O.D.) are evaluated for applications in NMR spectroscopy. The coils are first overfilled with a standard sucrose solution and compared against each another. The coils with smaller I.D. (≤ 100 µm) perform extremely well. One hypothesis is that as the coils get smaller the volume occupied by the copper turns increases relative to the open I.D, as such a large proportion of sample is brought in close proximity to the coil turns and likely gives strong sample-coil magnetic coupling which increases signal. Applications of the planar microcoils are demonstrated on a Cypselurus poecilopterus (fish) and Daphnia magna (water flea) eggs. A single D. magna egg on a 50 μm coil yielded at least 3,000 times the mass sensitivity (~9,000,000 time saving) when compared to a 5-mm probe. This value could be at least 4 times higher if the B1 homogeneity of the coils could be improved. With the current design, 80% of the signal is lost in multiple pulse experiments that rely on phased inversion and signal cancellation between scans. The data was extrapolated to predict that biological samples as small as ~4 μm may become accessible via planar microcoil designs. To fulfill their potential for in situ metabolic screening, specialized magnetic suspectibility matched sample holders that restrict the sample to the homogenious B1 field region (i.e. within the 90% RF field) of the coil and advanced experiments that narrow spectral lines, suppress lipids and disperse signals into multiple dimensions, will be required.

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Publication details

The article was received on 11 Aug 2017, accepted on 03 Nov 2017 and first published on 03 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01339F
Citation: Analyst, 2017, Accepted Manuscript
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    Towards Single Egg Toxicity Screening Using Microcoil NMR

    I. Fugariu, R. Soong , D. Lane, M. Fey, W. Maas, F. Vincent, A. Beck, D. Schmidig, B. Treanor and A. Simpson, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01339F

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