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Issue 19, 2017
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A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(II) complexes for heparin detection

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Abstract

A sensitive assay was demonstrated for the colorimetric and luminescent detection of heparin. Positively charged [Ru(phen)2np]2+ (np: naphtho[2,3-f][1,10]phenanthroline) can interact with negatively charged AuNPs through electrostatic interaction, leading to the luminescence quenching of [Ru(phen)2np]2+via energy transfer as well as the aggregation of AuNPs together with a color change in solution from red to blue. Upon the addition of negatively charged heparin to gold nanoparticles functionalized by ruthenium(II) complexes, the emission of [Ru(phen)2np]2+ recovered due to the competitive adsorption of [Ru(phen)2np]2+ to heparin and AuNPs. A linear calibration curve was obtained in the 0.4 μM to 100 μM range and 0.1 μM to 6 μM with a low detection limit of 0.22 μM and 0.024 μM based on 3σ/s for heparin quantification in buffer solution and in 1% fetal bovine serum (FBS), respectively. Interestingly, the naked eye alone can judge the presence of 0.02 μM heparin without the aid of any advanced instruments by color change. Thus a convenient and visual method to detect heparin was established.

Graphical abstract: A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(ii) complexes for heparin detection

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Publication details

The article was received on 29 Jul 2017, accepted on 15 Aug 2017 and first published on 16 Aug 2017


Article type: Paper
DOI: 10.1039/C7AN01253E
Citation: Analyst, 2017,142, 3733-3739
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    A luminescent and colorimetric probe based on the functionalization of gold nanoparticles by ruthenium(II) complexes for heparin detection

    J. Li, M. Cheng and M. Li, Analyst, 2017, 142, 3733
    DOI: 10.1039/C7AN01253E

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