Jump to main content
Jump to site search


An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA-RNA hybridization

Abstract

In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA-RNA hybridization configuration. A biotinylated miRNA of selfsame sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto tungsten diselenide (WSe2) nanosheets modified electrode. Whereafter, the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin-horseradish peroxidase (HRP) conjugates to catalyze H2O2+hydroquinone (HQ) system. Benefiting from high specific surface area of WSe2 nanosheets, competitive hybridization configuration and signal amplification of H2O2+HQ detection system, the proposed assay exhibits a wide linear range of 0.0001-100 pM towards target miRNA with a detection limit of 0.06 fM (S/N=3), and shows excellent distinction ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for detection of miRNA in biomedical research and early clinical diagnosis.

Back to tab navigation

Publication details

The article was received on 27 Jul 2017, accepted on 01 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01244F
Citation: Analyst, 2017, Accepted Manuscript
  •   Request permissions

    An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA-RNA hybridization

    Y. Chen, W. Zhang, K. Huang, M. Zheng and Y. Mao, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01244F

Search articles by author

Spotlight

Advertisements