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An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization

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Abstract

In this work, we report an ultrasensitive electrochemical biosensor for microRNA-21 (miRNA-21) detection by using a competitive RNA–RNA hybridization configuration. A biotinylated miRNA of the self-same sequence with the target miRNA is mixed with the samples, and allowed competition with the target miRNA for a thiolated RNA probe immobilized onto a tungsten diselenide (WSe2) nanosheet modified electrode. Thereafter the current response is obtained by forming the hybridized biotinylated miRNA with streptavidin–horseradish peroxidase (HRP) conjugates to catalyze the H2O2 + hydroquinone (HQ) system. Benefiting from the high specific surface area of WSe2 nanosheets, the competitive hybridization configuration and the signal amplification of the H2O2 + HQ detection system, the proposed assay exhibits a wide linear range of 0.0001–100 pM towards target miRNA with a detection limit of 0.06 fM (S/N = 3), and shows excellent discrimination ability for base-mismatched miRNA sequences. Therefore, the designed platform has promising prospects for the detection of miRNA in biomedical research and early clinical diagnosis.

Graphical abstract: An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization

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Publication details

The article was received on 27 Jul 2017, accepted on 01 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7AN01244F
Citation: Analyst, 2017, Advance Article
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    An electrochemical microRNA sensing platform based on tungsten diselenide nanosheets and competitive RNA–RNA hybridization

    Y. Chen, W. Zhang, K. Huang, M. Zheng and Y. Mao, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN01244F

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