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New lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for tetrodotoxin rapid detection

Abstract

Tetrodotoxin (TTX) is a potent, low molecular weight analyte that can lead to fatal poisoning and requires a sensitive, rapid detection method. Here, we have developed a competitive, lateral-flow immunochromatographic strip combined with quantum dot nanobeads (QDNBs) and gold nanoflowers (AuNFs). This approach is called turn-on C-LFICS termed and meets all testing requirements. Subsequent analysis revealed that this turn-on C-LFICS was rapid (8 min), sensitive (LOD=0.2 ng/mL), quantitative (DLR=1.56-100 ng/mL), and had a positive signal readout (based on fluorescence quenching effects) for TTX detection. Moreover, it had superior signal brightness and low background interference signal when compared with previous methods. Finally, it can function free of interference from sample matrix and has a demonstrated recovery range of 85.5% to 119.7% in spiked samples. Taken together, these results show that our turn-on C-LFICS is an effective detection tool for TTX or other small molecules.

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Supplementary files

Publication details

The article was received on 24 Jul 2017, accepted on 10 Oct 2017 and first published on 11 Oct 2017


Article type: Paper
DOI: 10.1039/C7AN01227F
Citation: Analyst, 2017, Accepted Manuscript
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    New lateral-flow immunochromatographic strip combined with quantum dot nanobeads and gold nanoflowers for tetrodotoxin rapid detection

    H. Shen, F. Xu, M. Xiao, Q. Fu, Z. Cheng, S. Zhang, C. Huang and Y. Tang, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01227F

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