Jump to main content
Jump to site search


Multiplexed detection of microRNAs by a competitive DNA microarray-based resonance light scattering assay

Abstract

The reliable detection and monitoring of microRNAs (miRNAs) have great significance for gaining a better understanding of miRNAs’ function in a wide range of biological processes. In this work, a competitive DNA microarray-based resonance light scattering (RLS) assay has been developed for multiplexed detection of miRNAs with relatively high sensitivity and selectivity. After one-step competition hybridization reactions of the miRNAs and multiple single strand DNAs (ssDNAs) conjugated gold nanoparticles (ssDNAsn@GNPs) with immobilized ssDNA probes on the dendrimer-modified slide, the captured ssDNAsn@GNPs are further enlarged through silver deposition. The silver enlarged ssDNAsn@GNPs can generate strong RLS under white light excitation. Simultaneous detection of multiple miRNAs can be easily achieved by monitoring the RLS signal changes of microarrays. In the proof of concept experiment, eight miRNA let-7 family members are detected with high specificity down to 0.2 pM, 0.8 pM, 0.2 pM, 0.2 pM, 0.2 pM, 0.8 pM, 0.8 pM, 0.8 pM for miRNA let-7a to 7g, and 7i, respectively. Furthermore, expression levels of eight miRNA let-7 family members in the total RNA extracts from five cell lines have been evaluated, and satisfactory results are obtained.

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Jul 2017, accepted on 10 Oct 2017 and first published on 12 Oct 2017


Article type: Paper
DOI: 10.1039/C7AN01152K
Citation: Analyst, 2017, Accepted Manuscript
  •   Request permissions

    Multiplexed detection of microRNAs by a competitive DNA microarray-based resonance light scattering assay

    X. Liu, R. Tian, J. Gao, D. Liu and Z. Wang, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01152K

Search articles by author

Spotlight

Advertisements