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A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten

Abstract

The development of multianalyte immunoassays constitutes a main research issue in the field of bioanalytical techniques. In the present study, class-specific antibodies against the three members of the anilinopyrimidine family of fungicides (pyrimethanil, cyprodinil, and mepanipyrim) were raised by using a bioconjugate of a rationally designed hapten [5-(6-methyl-2-(phenylamino)pyrimidin-4-yl)pentanoic acid]. Highly sensitive immunoassays were developed for the generic determination of these compounds, using the competitive enzyme-linked immunosorbent assay (ELISA). Particularly, a direct antibody-coated competitive ELISA afforded identical sensitivity for the three anilinopyrimidines, with IC50 values of 0.26, 0.27, and 0.25 μg L−1 for pyrimethanil, cyprodinil, and mepanipyrim, respectively. This immunoassay was fully characterized and applied to the multianalyte determination of anilinopyrimidine fungicides in white and red wines, with a limit of quantification of 1 μg L−1, average recoveries from 93.1% to 114.4%, and relative standard deviations lower than 20%. Commercial wine samples were analyzed and those containing detectable anilinopyrimide residues were verified by a reference chromatographic technique.

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Publication details

The article was received on 05 Jul 2017, accepted on 14 Sep 2017 and first published on 14 Sep 2017


Article type: Paper
DOI: 10.1039/C7AN01138E
Citation: Analyst, 2017, Accepted Manuscript
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    A class-selective immunoassay for simultaneous analysis of anilinopyrimidine fungicides using a rationally designed hapten

    F. A. Esteve-Turrillas, J. V. Mercader, C. Agulló, A. Abad-Somovilla and A. Abad-Fuentes, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01138E

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