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Developing a combined strategy for monitoring the progress of aptamer selection

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Abstract

The development of a simple, low-cost, time-saving and universally applicable method to monitor the progression of aptamer selection is particularly challenging. Herein, a combined strategy dependent on quantitative polymerase chain reaction amplification curve (AC) and melting curve analysis (MCA) is developed to monitor the convergence of the aptamer species during selection progress. As a parallel and complementary method to affinity tests and binding analyses, the AC-MCA method can be used to achieve the DNA complexity assay when affinity is undetectable. It is independent of the target properties and SELEX methods. Therefore, it has been demonstrated as a universal monitoring tool in different SELEX methods towards different targets (small molecules, proteins, bacteria and cancer cells). The AC-MCA method will facilitate current new aptamer discovery and aptamer-based wide application.

Graphical abstract: Developing a combined strategy for monitoring the progress of aptamer selection

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Publication details

The article was received on 05 Jul 2017, accepted on 24 Jul 2017 and first published on 26 Jul 2017


Article type: Communication
DOI: 10.1039/C7AN01131H
Citation: Analyst, 2017, Advance Article
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    Developing a combined strategy for monitoring the progress of aptamer selection

    Z. Luo, L. He, J. Wang, X. Fang and L. Zhang, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN01131H

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