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Bioresponsive-controlled release of methylene blue from magnetic mesoporous silica from electrochemical detection of telomerase activity

Abstract

An electrochemical sensing platform was designed to monitor the telomerase activity in HeLa cell, using bioresponsive-controlled cargo release from magnetic mesoporous silica nanocontainers (MMSNs). The aminated MMSNs were first synthesized by a wet-chemistry method, then methylene blue (indicator) molecules were loaded into the pores with the aid of specifially designed wrapping DNA strands, and then the wrapping DNA-gated MMSNs were immobilized on a magnetic removable screen-printing carbon electrode. Upon target telomerase and dNTPs introduction in the detection cell, the wrapping DNA strands on the MMSNs were prolonged to form rigid hairpin-like DNA structures, thus resulting in the dissociation of wrapping DNA strands from the MMSNs. Thereafter, the loaded methylene blue with redox activity were released out from the pores, thereby causing the increasing electrochemical signal relative to the background signal. Under optimal conditions, MMSN-based sensing system exhibited good voltammetric responses toward target telomerase activity within the dynamic linear range of 50 – 5000 cells mL-1 at a detection limit of 12 cells mL-1 in the HeLa extract. The reproducibility and generality of our strategy were acceptable by using somatic tumor cell lines. In addition, the inhibition effect of this system was also evaluated by using 3'-azido-3'-deoxythymidine as a telomerase inhibitor, receiving good results in this screening research.

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Publication details

The article was received on 05 Jul 2017, accepted on 28 Jul 2017 and first published on 01 Aug 2017


Article type: Paper
DOI: 10.1039/C7AN01127J
Citation: Analyst, 2017, Accepted Manuscript
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    Bioresponsive-controlled release of methylene blue from magnetic mesoporous silica from electrochemical detection of telomerase activity

    M. Lu, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN01127J

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