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Simple and rapid colorimetric detection of melanoma circulating tumor cells using bifunctional magnetic nanoparticles

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Abstract

Circulating tumor cells (CTCs) are a promising biomarker for monitoring cancer metastasis and therapy response. However, the rarity and inherent fragility of CTCs pose great challenges for CTC detection. Currently, CTC detection requires advanced instruments that might be inaccessible in most hospitals or labs. To allow simple and rapid CTC detection, we propose a sensor platform that relies only on the intrinsic properties of magnetic nanoparticles (MNPs) including rapid separation as well as nanozyme activity. The detection of melanoma CTC, which causes most skin cancer-related deaths, is demonstrated based on bifunctional MNPs. The magnetic property of MNPs enables melanoma CTC isolation and enrichment within 5 min from blood. The nanozyme activity of MNPs allows naked-eye detection of melanoma CTC by catalyzing the oxidation of colourless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue coloured products. Melanoma CTC enumeration can be further achieved by UV-vis measurement. Our platform has successfully detected 13 melanoma CTCs per mL within 50 min. We thus believe this platform could find broad applications both in the clinic and in research.

Graphical abstract: Simple and rapid colorimetric detection of melanoma circulating tumor cells using bifunctional magnetic nanoparticles

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Publication details

The article was received on 02 Jul 2017, accepted on 25 Oct 2017 and first published on 26 Oct 2017


Article type: Paper
DOI: 10.1039/C7AN01102D
Citation: Analyst, 2017, Advance Article
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    Simple and rapid colorimetric detection of melanoma circulating tumor cells using bifunctional magnetic nanoparticles

    J. Li, J. Wang, Y. Wang and M. Trau, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN01102D

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