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Applications of vibrational tags in biological imaging by Raman microscopy

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Abstract

As a superb tool to visualize and study the spatial-temporal distribution of chemicals, Raman microscopy has made a big impact in many disciplines of science. While label-free imaging has been the prevailing strategy in Raman microscopy, recent development and applications of vibrational/Raman tags, particularly when coupled with stimulated Raman scattering (SRS) microscopy, have generated intense excitement in biomedical imaging. SRS imaging of vibrational tags has enabled researchers to study a wide range of small biomolecules with high specificity, sensitivity and multiplex capability, at a single live cell level, tissue level or even in vivo. As reviewed in this article, this platform has facilitated imaging distribution and dynamics of small molecules such as glucose, lipids, amino acids, nucleic acids, and drugs that are otherwise difficult to monitor with other means. As both the vibrational tags and Raman instrumental development progress rapidly and synergistically, we anticipate that this technique will shed light onto an even broader spectrum of biomedical problems.

Graphical abstract: Applications of vibrational tags in biological imaging by Raman microscopy

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Publication details

The article was received on 15 Jun 2017, accepted on 10 Aug 2017 and first published on 15 Aug 2017


Article type: Critical Review
DOI: 10.1039/C7AN01001J
Citation: Analyst, 2017, Advance Article
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    Applications of vibrational tags in biological imaging by Raman microscopy

    Z. Zhao, Y. Shen, F. Hu and W. Min, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN01001J

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