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Surface acoustic wave biosensor synergizing DNA-mediated in-situ silver nanoparticle growth for highly specific and signal-amplified nucleic acid assay

Abstract

This work reports a surface acoustic wave (SAW) DNA sensor that synergizes the surface mass effect for signal-amplified and sequence-specific DNA detection in blood serum. By combining an enzyme-mediated DNA extension reaction (both viscoelastic and mass fraction) with in-situ synthesis of silver nanoparticles (mass fraction), a highly sensitive SAW biosensing interface with synergic mass loading was tailor-engineered. As target DNA hybridized with the surface-confined capture probes, the exposed 3'-OH terminal of the target sequence could be triggered to elongate in the coexistence of terminal deoxynucleoside transferase (TdT) and deoxy-ribonucleoside triphosphate (dNTP), thereby producing an evident mass effect. Importantly, the extended domain can serve as a template to specifically hybridize with Ag+-binding sequences. In the presence of reducing agents, the accumulated silver ions would nucleate for in-situ synthesis of silver nanoparticles, further enhancing the mass loading. By using this approach, we observed a rapid growing event of silver nanoparticles for signal enhancement, which improved the detection limit (0.8 pM) of the SAW sensor by 3 orders of magnitude as compared to the strategy without signal amplification (at nanomolar level). The sensor also achieved a high specificity in discriminating even single-mismatched DNA sequence, and meanwhile could probe the low-abundance DNA molecules directly in human serum with minimal interference. These advantages make the SAW biosensor promising for practical applications, such as monitoring of molecular interactions and disease diagnostics.

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Publication details

The article was received on 14 Jun 2017, accepted on 01 Aug 2017 and first published on 04 Aug 2017


Article type: Paper
DOI: 10.1039/C7AN00988G
Citation: Analyst, 2017, Accepted Manuscript
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    Surface acoustic wave biosensor synergizing DNA-mediated in-situ silver nanoparticle growth for highly specific and signal-amplified nucleic acid assay

    Y. Zhang, F. Yang, Z. Sun, Y. Li and G. Zhang, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00988G

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