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Surface plasmon field-enhanced fluorescence reversible split aptamer biosensor

Abstract

Surface plasmon field-enhanced fluorescence is reported for the readout of a heterogeneous assay that utilizes low affinity split aptamer ligand. Weak affinity ligands that reversibly interact with target analyte hold potential for facile implementation in continuous monitoring biosensor systems. This functionality is not possible without the regeneration for more commonly used assays relying on high affinity ligands and end-point measurement. In fluorescence-based sensors, the use of low affinity ligands allows avoiding this step but it imposes a challenge associated with the weak optical response to the specific capture of target analyte which is also often masked by a strong background. The coupling of fluorophore labels with confined field of surface plasmons is reported for strong amplification of fluorescence signal emitted from the sensor surface and its efficient discrimination from the background. This optical scheme is demonstrated for time-resolved analysis of chosen model analyte – adenoside and adenosine triphosphate – with a split aptamer that exhibits equlibrium affinity binding constant between 0.73 and 1.35 mM. The developed biosensor allowed for rapid specific discrimination of target analyte concentration changes from low µM to mM in several minutes in buffer as well as in 10% serum.

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Supplementary files

Publication details

The article was received on 10 Jun 2017, accepted on 06 Jul 2017 and first published on 07 Jul 2017


Article type: Paper
DOI: 10.1039/C7AN00970D
Citation: Analyst, 2017, Accepted Manuscript
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    Surface plasmon field-enhanced fluorescence reversible split aptamer biosensor

    K. Sergelen, B. Liedberg, W. Knoll and J. Dostalek, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00970D

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