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Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS

Abstract

Protein methylation is an important post-translational modification (PTM) that plays crucial roles in the regulation of diverse biological processes. Though many efforts have been devoted to the investigation of protein methylation, the analysis of non-histone methylation at proteome level is still a great challenge. The alteration of protein/peptide physicochemical properties caused by methylation is very small, thus it is difficult to develop highly efficient enrichment approaches to separate the methylated peptides from the pool of diverse background peptides. The mass shifts caused by methylations are identical to the substitutions of some amino acids, thus it is difficult to confidently identify methylated peptides. In this review, we will introduce the recent advances in the method development for the large-scale analysis of non-histone protein methylation. Especially the methods for efficient enrichment and the approaches for controlling identification confidence will be covered.

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Publication details

The article was received on 09 Jun 2017, accepted on 08 Aug 2017 and first published on 08 Aug 2017


Article type: Critical Review
DOI: 10.1039/C7AN00954B
Citation: Analyst, 2017, Accepted Manuscript
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    Strategies for large-scale analysis of non-histone protein methylation by LC-MS/MS

    Q. Wang, K. Wang and M. Ye, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00954B

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