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Issue 21, 2017
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A multi-functional guanine derivative for studying the DNA G-quadruplex structure

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Abstract

In the present study, we developed a multi-functional guanine derivative, 8FG, as a G-quadruplex stabilizer, a fluorescent probe for the detection of G-quadruplex formation, and a 19F sensor for the observation of the G-quadruplex. We demonstrate that the functional nucleoside bearing a 3,5-bis(trifluoromethyl)benzene group at the 8-position of guanine stabilizes the DNA G-quadruplex structure and fluoresces following the G-quadruplex formation. Furthermore, we show that the functional sensor can be used to directly observe DNA G-quadruplexes by 19F-NMR in living cells. To our knowledge, this is the first study showing that the nucleoside derivative simultaneously allows for three kinds of functions at a single G-quadruplex DNA. Our results suggest that the multi-functional nucleoside derivative can be broadly used for studying the G-quadruplex structure and serves as a powerful tool for examining the molecular basis of G-quadruplex formation in vitro and in living cells.

Graphical abstract: A multi-functional guanine derivative for studying the DNA G-quadruplex structure

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Publication details

The article was received on 07 Jun 2017, accepted on 28 Aug 2017 and first published on 01 Sep 2017


Article type: Paper
DOI: 10.1039/C7AN00941K
Citation: Analyst, 2017,142, 4083-4088
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    A multi-functional guanine derivative for studying the DNA G-quadruplex structure

    T. Ishizuka, P. Zhao, H. Bao and Y. Xu, Analyst, 2017, 142, 4083
    DOI: 10.1039/C7AN00941K

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