Jump to main content
Jump to site search


A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis

Author affiliations

Abstract

A single-round DNA aptamer selection for mammalian cells was successfully achieved for the first time using a capillary electrophoresis (CE)-based methodology called polymer-enhanced capillary transient isotachophoresis (PectI). The PectI separation yielded a single peak for the human lung cancer cell line (PC-9) complexed with DNA aptamer candidates, which was effectively separated from a free randomized DNA library peak, ensuring no contamination from free DNA in the PC-9–DNA aptamer complex fraction. The DNA aptamer candidates obtained after a single-round selection employing counter selection with HL-60 were proven to bind selectively and form kinetically stable complexes with PC-9 cells. Interestingly, most aptamer candidates showed high binding ability (Kd = 70–350 nM) with different extents of binding on the cell surface. These facts proved that a single-round selection for mammalian cells by PectI is feasible to obtain various types of aptamer candidates, which have high-affinity even for non-overexpressed but unique targets on the cell surface in addition to overexpressed targets.

Graphical abstract: A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Jun 2017, accepted on 15 Aug 2017 and first published on 15 Aug 2017


Article type: Paper
DOI: 10.1039/C7AN00909G
Citation: Analyst, 2017, Advance Article
  •   Request permissions

    A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis

    K. Hirose, M. Tsuchida, H. Asakura, K. Wakui, K. Yoshimoto, K. Iida, M. Sato, M. Shibukawa, M. Suganuma and S. Saito, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00909G

Search articles by author

Spotlight

Advertisements