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Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches

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Abstract

In the present Communication, we propose a quite simple but previously overlooked approach for conveniently analyzing, assigning, and extracting sialic acid-containing N-glycan structures using high-resolution NMR spectroscopy without pre-installing metal chelators. Paramagnetic metals, such as Gd3+, appear to bind to the carboxyl groups of N-acetylneuraminic acid when introduced at room temperature, leading to the measurement of nonequivalent proton and carbon NMR spectral signals among otherwise “identical” glycan branched structures.

Graphical abstract: Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches

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Publication details

The article was received on 17 May 2017, accepted on 05 Jul 2017 and first published on 07 Jul 2017


Article type: Communication
DOI: 10.1039/C7AN00817A
Citation: Analyst, 2017, Advance Article
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    Simple Gd3+-Neu5NAc complexation results in NMR chemical shift asymmetries of structurally equivalent complex-type N-glycan branches

    K. Usachev, Y. Yamaguchi, M. Takamatsu, N. Pavlova, V. Klochkov, A. Kurbangalieva, T. Murase, T. Shimoda and K. Tanaka, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00817A

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