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Effect of Mg and Sr onto the crystallinity of bones evaluated through Raman spectroscopy and laser ablation-ICPMS analysis

Abstract

We investigated the possible linkage between crystallinity and elemental ratios (Mg/Ca and Sr/Ca) of femoral cortical bones of the rats with chronic kidney disease (CKD) or diabetes mellitus (DM). The Mg/Ca and Sr/Ca ratios were measured by a laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) technique and the crystallinity was evaluated by a Raman spectroscopy on the same sample slice. The measured crystallinity varied significantly along the radial direction, reflecting the heterogeneities in Mg/Ca ratio for the bone samples. For endosteal cortical bone of CKD rats, the Mg/Ca ratio became higher. This is explained by the increase of Mg abundance in bone, possibly due to the higher absorption efficiency of Mg at intestine or due to the lower excretion efficiency of Mg from kidney. For areas with the higher Mg/Ca ratio, crystallinity of bone was significantly degraded than those for areas with the lower Mg/Ca ratio, suggesting that the substitution of Ca by Mg induced the deterioration of crystallinity of hydroxyapatite (HAp). In addition, the measured Mg/Ca and Sr/Ca ratios of bone were positively correlated with those found in serum samples. Data obtained here demonstrated that the metabolic alterations for inorganic elements caused the ionic substitutions of Ca by cationic foreign ions, and the contents of foreign ions in bone greatly affected the crystallinity of HAp.

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Supplementary files

Publication details

The article was received on 03 May 2017, accepted on 04 Sep 2017 and first published on 07 Sep 2017


Article type: Paper
DOI: 10.1039/C7AN00734E
Citation: Analyst, 2017, Accepted Manuscript
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    Effect of Mg and Sr onto the crystallinity of bones evaluated through Raman spectroscopy and laser ablation-ICPMS analysis

    Y. Tanaka, N. Yajima, M. Okada, T. Matsumoto, Y. Higuchi, S. Miyazaki, H. Yamato and T. Hirata, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00734E

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