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Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

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Abstract

We use single molecule spectroscopy to study a multicomponent, competitive protein adsorption system. Fluorescently-labeled α-lactalbumin proteins are super-resolved adsorbing to cationic anion-exchange ligands in the presence of a competitor, insulin. We find that the competitor reduces the number of binding events by blocking ligands throughout the observed measurement time while the single-site adsorption kinetics are unchanged.

Graphical abstract: Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

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Publication details

The article was received on 27 Apr 2017, accepted on 14 Jul 2017 and first published on 18 Jul 2017


Article type: Communication
DOI: 10.1039/C7AN00701A
Citation: Analyst, 2017, Advance Article
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    Competitive multicomponent anion exchange adsorption of proteins at the single molecule level

    L. Kisley, U. Patil, S. Dhamane, K. Kourentzi, L. J. Tauzin, R. C. Willson and C. F. Landes, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00701A

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