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Simultaneous label-free screening of G-quadruplex active ligands from natural medicine with a microfluidic chip electrophoresis-based energy transfer multi-biosensor strategy

Abstract

Rapid screening of active compounds plays a crucial role in the research and application of complex natural medicine. A new method of simultaneous label-free multi-drug screening based on selective aptamer-carboxyfluorescein/graphene oxide energy transfer optical sensor combined with microfluidic chip electrophoretic separation was reported here. Seven traditional Chinese medicinal monomers were chosen as targets for the screening of G-quadruplex ligands. The screening results of G-quadruplex active ligands including daidzein, berberine hydrochloride, jatrorrhizine hydrochloride and fangchinoline, and non-active ligands including geniposide and oxymatrine were consistent with literature. At the same time, one new potential G4DNA active drug, jujuboside A, was identified. Molecular simulation of the interaction between G4DNA and drugs with Hyperchem and Autodock was also carried out to verify the results of the experimental screening. It further demonstrated the reliability of our strategy. This novel separation based multi-sensor strategy provides a simple, rapid, and sensitive tool for simultaneous multi-drug screening which is very meaningful for the drug screening and bio-interaction analysis.

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Publication details

The article was received on 26 Apr 2017, accepted on 02 Aug 2017 and first published on 04 Aug 2017


Article type: Paper
DOI: 10.1039/C7AN00692F
Citation: Analyst, 2017, Accepted Manuscript
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    Simultaneous label-free screening of G-quadruplex active ligands from natural medicine with a microfluidic chip electrophoresis-based energy transfer multi-biosensor strategy

    Y. Lu, S. Yu, F. Lin, F. Lin, X. Zhao, L. Wu, Y. Miao, H. Li, Y. Deng and L. Geng, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00692F

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