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Methods of protein corona isolation for magnetic nanoparticles


Nanoparticles (NPs) in contact with a biological environment get covered by proteins among which some are loosely and some tightly bound. The later form a hard protein corona (HPC) known to determine their biological behavior. Therefore, in order to study biological behaviour of NPs one needs to start from the HPC. However, established methods and standards of HPC isolation are still missing. This is especially a challenge in the case of magnetic NPs which are one of the major branches of nanomedicine. Therefore, we developed a novel HPC isolation method, a multi-step centrifugation method (MSCM), for single-domain magnetic NPs. MSCM was applied on iron oxide NPs in interaction with human blood and lymph serums and with different dilution in triplicate. The analysis of the composition of the obtained HPCs’ showed the reproducibility of MSCM. This new method was as well compared with the existing magnetic separation method (MagSep) and a study of the obtained HPC allowed us to established the validity limits of MagSep and MSCM on only superparamagnetic NPs and on any single-domain magnetic NPs, respectively. Surprisingly, the HPCs obtained by these two isolation methods were quite different, up to 50 %, suggesting that only these proteins, which are found in HPCs of both isolation methods, are in fact real HPC.

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Publication details

The article was received on 18 Apr 2017, accepted on 15 Jun 2017 and first published on 15 Jun 2017

Article type: Paper
DOI: 10.1039/C7AN00646B
Citation: Analyst, 2017, Accepted Manuscript
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    Methods of protein corona isolation for magnetic nanoparticles

    D. Bonvin, D. Chiappe, M. Moniatte, H. Hofmann and M. Mionic Ebersold, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00646B

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