Issue 15, 2017

Immunoassay quantification using surface-enhanced fluorescence (SEF) tags

Abstract

Fluorescence-based immonoassays are widely used in several areas, ranging from basic biomedical research to disease diagnostics. A variety of new probes have been developed recently to address some limitations in typical assays performed with organic dyes. Ideally, new fluorescence tags that allow quantification with a low limit of detection are highly desired. In this work, the surface-enhanced fluorescence (SEF) phenomenon was explored in the development of tags for Immunoglobulin-M (IgM) detection. Shell-isolated gold nanoparticles (Au-SHINs) with 100 nm core size and a 10 nm silica shell were synthesized. These particles contain an outermost thin fluorescent layer of nile blue (NB) that was further coated by another 5 nm of silica (SEF tags). The outer silica shell was then functionalized with antibodies to allow the detection of IgM as in typical immunological sandwich assays. IgM concentrations down to the 10 ng mL−1 mark were successfully detected. A linear dependence between the average fluorescence intensity and the IgM concentration was found.

Graphical abstract: Immunoassay quantification using surface-enhanced fluorescence (SEF) tags

Supplementary files

Article information

Article type
Paper
Submitted
18 Apr 2017
Accepted
28 Jun 2017
First published
06 Jul 2017

Analyst, 2017,142, 2717-2724

Immunoassay quantification using surface-enhanced fluorescence (SEF) tags

S. A. Camacho, R. G. Sobral-Filho, P. H. B. Aoki, C. J. L. Constantino and A. G. Brolo, Analyst, 2017, 142, 2717 DOI: 10.1039/C7AN00639J

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements