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Issue 23, 2017
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Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis

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Abstract

In this work, we develop, fabricate, and characterize a plasmonic nanocone array surface enhanced Raman spectroscopy (SERS) substrate with a uniform enhancement factor on the micron scale for qualitative and quantiative cell and cell lysate analysis. This work demonstrates how SERS substrates can be used as cell-based biosensors given that the enhancement factor of the substrate is sufficient for Raman detection and that the uniformity is high over the applicable surface area. These requirements allow accurate and quantitative comparisons between nonuniform samples under varying biochemical conditions. We apply the developed SERS substrate for Raman measurements and mapping of HeLa cells and cell lysate. This method is used for identification of UV-induced damage and detection of nanomolar concentrations of methylated guanine spiked in cell lysate samples.

Graphical abstract: Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis

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Publication details

The article was received on 15 Apr 2017, accepted on 17 Oct 2017 and first published on 17 Oct 2017


Article type: Paper
DOI: 10.1039/C7AN00630F
Citation: Analyst, 2017,142, 4422-4430
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    Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis

    L. P. Hackett, L. L. Goddard and G. L. Liu, Analyst, 2017, 142, 4422
    DOI: 10.1039/C7AN00630F

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