Jump to main content
Jump to site search


Detection of a cancer biomarker protein on modified cellulose paper by fluorescence using aptamer-linked quantum dots

Author affiliations

Abstract

The development of point-of-care bioassays for sensitive screening of protein-based cancer biomarkers would improve the opportunity for early stage diagnosis. A strategy for a fluorescence resonance energy transfer (FRET)-based bioassay has been investigated that makes use of modified cellulose paper for the detection of an epithelial cell adhesion molecule (EpCAM), which is a transmembrane glycoprotein that is overexpressed in several tumors of epithelial origin. The paper matrix was a substrate for immobilized aptamer-linked quantum dots (QDs-Apt) and Cy3 labeled complementary DNA (cDNA), which served as a donor and an acceptor, respectively. Competitive binding of EpCAM displaced the cDNA, resulting in the reduction of FRET. The paper-based bioassay was able to detect EpCAM in buffer solution as well as in 10% bovine serum solution using a reaction time of no more than 60 minutes. The dynamic range was 1–100 nM in buffer with a precision better than 4%, and the limit of detection was 250 pM in buffer and 600 pM in 10% serum.

Graphical abstract: Detection of a cancer biomarker protein on modified cellulose paper by fluorescence using aptamer-linked quantum dots

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Apr 2017, accepted on 19 Jul 2017 and first published on 19 Jul 2017


Article type: Communication
DOI: 10.1039/C7AN00624A
Citation: Analyst, 2017, Advance Article
  •   Request permissions

    Detection of a cancer biomarker protein on modified cellulose paper by fluorescence using aptamer-linked quantum dots

    P. Das and U. J. Krull, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00624A

Search articles by author

Spotlight

Advertisements