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A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity

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Abstract

We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl−1 of DNA with no sequencing requirement.

Graphical abstract: A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity

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Publication details

The article was received on 11 Apr 2017, accepted on 15 Aug 2017 and first published on 16 Aug 2017


Article type: Communication
DOI: 10.1039/C7AN00611J
Citation: Analyst, 2017, Advance Article
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    A multiplex microplatform for the detection of multiple DNA methylation events using gold–DNA affinity

    A. A. I. Sina, M. T. Foster, D. Korbie, L. G. Carrascosa, M. J. A. Shiddiky, J. Gao, S. Dey and M. Trau, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00611J

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