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Facile detection of glucose in human serum employing silver-ion-guided surface-enhanced Raman spectroscopy signal amplification

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Abstract

A facile surface-enhanced Raman scattering (SERS) sensor based on a silver-ion-mediated amplification effect was designed for the determination of glucose concentration. In this approach, 4-aminothiophenol (4-ATP) molecules assembled on the surface of a gold wafer (Au wafer@4-ATP) act not only as Raman tags but also as linkage agents. Silver nanoparticles marked with cysteamine (AgNP@cys) were used as the SERS enhancement components because they could be bound to the Au wafer@4-ATP in the presence of silver ions through the formation of N → Ag+ ← N coordination compounds. Here, the Ag+ ions were obtained by using glucose oxidase to catalyze the oxidation of glucose, producing hydrogen peroxide (H2O2) to etch the AgNPs. Therefore, we recorded the SERS intensity of 4-ATP to determine the concentration of glucose in a phosphate buffer as low as 0.1 mM and further achieved a lowest detection of 0.5 mM glucose in human serum. These results show that the proposed approach has strong potential for practical applications.

Graphical abstract: Facile detection of glucose in human serum employing silver-ion-guided surface-enhanced Raman spectroscopy signal amplification

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Publication details

The article was received on 10 Apr 2017, accepted on 17 Jun 2017 and first published on 21 Jun 2017


Article type: Communication
DOI: 10.1039/C7AN00604G
Citation: Analyst, 2017, Advance Article
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    Facile detection of glucose in human serum employing silver-ion-guided surface-enhanced Raman spectroscopy signal amplification

    C. Fu, S. Jin, J. Oh, S. Xu and Y. M. Jung, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00604G

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