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K-junction structure mediated exponential signal amplification strategy for microRNA detection in electrochemiluminescence biosensor

Abstract

Based on the novel designed K-junction structure, an economic and efficient exponential signal amplification strategy with simple protocol combining hemin/G-quadruplex, a mimetic peroxidase, as catalyzer was proposed and utilized in an electrochemiluminescence biosensor for sensitive microRNA detection. It was noteworthy that the K-junction structure was formed with guanine-rich reporter DNA and substrate DNA modified by phosphate at 5'-terminus. Thus, the activity of reporter DNA could be inhibited and the recognition domain for target microRNA was constructed through the formed K-junction structure. When target microRNA sensed, paired domains of substrate DNA was completely digested from 5'-terminus, accompanied by the release of target microRNA and unpaired DNA fragment of substrate DNA called trigger DNA. Afterwards, the released microRNA and trigger DNA were recycled over and over with the digestion of K-junction structure linear and exponential grade respectively. As a result, numerous uninhibited reporter DNAs were left on the electrode lonely to capture hemin with hemin/G-quadruplex yielded which could enhance ECL emission significantly due to its catalysis for the luminol-H2O2. As expected, this method exhibited excellent specificity and high sensitivity for microRNA detection from down to 0.033 fM. What’s more, the application in human lung cancer cell lines achieved good sensitivity of 10 tumor cells.

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Publication details

The article was received on 08 Apr 2017, accepted on 02 May 2017 and first published on 08 May 2017


Article type: Paper
DOI: 10.1039/C7AN00586E
Citation: Analyst, 2017, Accepted Manuscript
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    K-junction structure mediated exponential signal amplification strategy for microRNA detection in electrochemiluminescence biosensor

    X. Li, A. Chen, Y. Chai and R. Yuan, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00586E

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