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Issue 15, 2017
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Monitoring focal adhesion kinase phosphorylation dynamics in live cells

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Abstract

Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.

Graphical abstract: Monitoring focal adhesion kinase phosphorylation dynamics in live cells

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Publication details

The article was received on 18 Mar 2017, accepted on 24 May 2017 and first published on 24 May 2017


Article type: Communication
DOI: 10.1039/C7AN00471K
Citation: Analyst, 2017,142, 2713-2716
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    Monitoring focal adhesion kinase phosphorylation dynamics in live cells

    N. P. Damayanti, K. Buno, N. Narayanan, S. L. Voytik Harbin, M. Deng and J. M. K. Irudayaraj, Analyst, 2017, 142, 2713
    DOI: 10.1039/C7AN00471K

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