Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.



Real time plasmonic qPCR: how fast is ultra-fast? 30 cycles in 54 seconds

Author affiliations

Abstract

Polymerase Chain Reaction (PCR) is a critical tool for biological research investigators but recently it also has been making a significant impact in clinical, veterinary and agricultural applications. Plasmonic PCR, which employs the very efficient heat transfer of optically irradiated metallic nanoparticles, is a simple and powerful methodology to drive PCR reactions. The scalability of next generation plasmonic PCR technology will introduce various forms of PCR applications ranging from small footprint portable point of care diagnostic devices to large footprint central laboratory multiplexing devices. In a significant advance, we have introduced a real time plasmonic PCR and explored the ability of ultra-fast cycling compatible with both label-free and fluorescence-based monitoring of amplicon production. Furthermore, plasmonic PCR has been substantially optimized to now deliver a 30 cycle PCR in 54 seconds, with a detectable product. The advances described here will have an immediate impact on the further development of the use of plasmonic PCR playing a critical role in rapid point of care diagnostics.

Graphical abstract: Real time plasmonic qPCR: how fast is ultra-fast? 30 cycles in 54 seconds

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 20 Feb 2017, accepted on 12 Apr 2017 and first published on 12 Apr 2017


Article type: Paper
DOI: 10.1039/C7AN00304H
Citation: Analyst, 2017, Advance Article
  •   Request permissions

    Real time plasmonic qPCR: how fast is ultra-fast? 30 cycles in 54 seconds

    P. J. R. Roche, M. Najih, S. S. Lee, L. K. Beitel, M. L. Carnevale, M. Paliouras, A. G. Kirk and M. A. Trifiro, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00304H

Search articles by author