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Real Time Plasmonic qPCR: How fast is Ultra-fast? 30 cycles in 54 seconds.

Abstract

Polymerase Chain Reaction (PCR) is a critical tool for biological research investigators but recently it also has been making a significant impact in clinical, veterinary and agricultural applications. Plasmonic PCR, which employs very efficient heat transfer of optically irradiated metallic nanoparticles, is a simple and powerful methodology to drive PCR reactions. The scalability of next generation plasmonic PCR technology will introduce various forms of PCR applications ranging from small footprint portable point of care diagnostic devices to large footprint central laboratory multiplexing devices. In a significant advance, we have introduced a real time plasmonic PCR and explored the ability of ultra-fast cycling compatible with both label-free, and fluorescence-based monitoring of amplicon production. Furthermore, plasmonic PCR has been substantially optimized to now deliver a 30 cycle PCR in 54 seconds, with a detectable product. The advances described here will have an immediate impact on the further development of the use of plasmonic PCR playing a critical role in rapid point of care diagnostics.

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Publication details

The article was received on 20 Feb 2017, accepted on 12 Apr 2017 and first published on 12 Apr 2017


Article type: Paper
DOI: 10.1039/C7AN00304H
Citation: Analyst, 2017, Accepted Manuscript
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    Real Time Plasmonic qPCR: How fast is Ultra-fast? 30 cycles in 54 seconds.

    P. J. R. Roche, M. Najih, S. Lee, L. K. Beitel, M. L. Carnevale, M. Paliouras, A. Kirk and M. Trifiro, Analyst, 2017, Accepted Manuscript , DOI: 10.1039/C7AN00304H

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