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Micro/extended-nano sampling interface from a living single cell

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Abstract

Single-cell analysis is of increasing importance in many fields, but is challenging due to the ultra-small volumes (picoliters) of single cells. Indeed, analysis of a specific analyte might require the analysis of a single molecule or several molecules. Analytical processes usually include sampling, chemical processing, and detection. Although several papers have reported chemical processing and detection methods for single cells, a sampling method compatible with maintaining the viability of a single cell during sampling has yet to be developed. Here, we propose a femtoliter sampling method from a living single cell using micro/nanofluidic device technology. The sampling of 39 fL of cytoplasm from a single human aortic endothelial cell was demonstrated and its viability after sampling was confirmed.

Graphical abstract: Micro/extended-nano sampling interface from a living single cell

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Publication details

The article was received on 07 Feb 2017, accepted on 30 Mar 2017 and first published on 10 Apr 2017


Article type: Paper
DOI: 10.1039/C7AN00220C
Citation: Analyst, 2017, Advance Article
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    Micro/extended-nano sampling interface from a living single cell

    L. Lin, K. Mawatari, K. Morikawa, Y. Pihosh, A. Yoshizaki and T. Kitamori, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00220C

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