Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.

Micro/extended-nano sampling interface from a living single cell

Author affiliations


Single-cell analysis is of increasing importance in many fields, but is challenging due to the ultra-small volumes (picoliters) of single cells. Indeed, analysis of a specific analyte might require the analysis of a single molecule or several molecules. Analytical processes usually include sampling, chemical processing, and detection. Although several papers have reported chemical processing and detection methods for single cells, a sampling method compatible with maintaining the viability of a single cell during sampling has yet to be developed. Here, we propose a femtoliter sampling method from a living single cell using micro/nanofluidic device technology. The sampling of 39 fL of cytoplasm from a single human aortic endothelial cell was demonstrated and its viability after sampling was confirmed.

Graphical abstract: Micro/extended-nano sampling interface from a living single cell

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 07 Feb 2017, accepted on 30 Mar 2017 and first published on 10 Apr 2017

Article type: Paper
DOI: 10.1039/C7AN00220C
Citation: Analyst, 2017, Advance Article
  •   Request permissions

    Micro/extended-nano sampling interface from a living single cell

    L. Lin, K. Mawatari, K. Morikawa, Y. Pihosh, A. Yoshizaki and T. Kitamori, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00220C

Search articles by author