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Rapid single-cell detection and identification of pathogens by using surface-enhanced Raman spectroscopy

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Abstract

For the successful treatment of infections, real-time analysis and enhanced multiplex capacity, sensitivity and cost-effectiveness of the developed detection method are critical. In this work, surface-enhanced Raman scattering (SERS) was employed with the final aim of identification and discrimination of pathogenic bacteria, based on their detected SERS fingerprint at the single-cell level. Several genera of bacteria that are found in most of the isolated infections in bacteraemia were successfully identified in less than 5 minutes without the use of antibodies or other specific receptors. The key element of the SERS direct detection platform is the SERS substrate, which combines easy production at low costs with a high enhancement enabling single-cell detection. The innovative approach of detection required the in situ synthesis of silver nanoparticles (NPs), ensuring an intimate contact with the bacterial membrane. This protocol provided a good reproducibility of the single-cell SERS spectra and was successfully applied both on Gram-negative and Gram-positive microorganisms (E. coli, M. morganii, E. lactis, L. casei). Thus, a label-free SERS-based biosensor for pathogen detection was developed with low costs, minimal sample preparation, high-accuracy and a very short analysis time of less than 5 min, which is crucial for infection diagnosis.

Graphical abstract: Rapid single-cell detection and identification of pathogens by using surface-enhanced Raman spectroscopy

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Publication details

The article was received on 19 Jan 2017, accepted on 26 Mar 2017 and first published on 28 Mar 2017


Article type: Paper
DOI: 10.1039/C7AN00106A
Citation: Analyst, 2017, Advance Article
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    Rapid single-cell detection and identification of pathogens by using surface-enhanced Raman spectroscopy

    N. E. Dina, H. Zhou, A. Colniţă, N. Leopold, T. Szoke-Nagy, C. Coman and C. Haisch, Analyst, 2017, Advance Article , DOI: 10.1039/C7AN00106A

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