Jump to main content
Jump to site search

Issue 7, 2017
Previous Article Next Article

Tunable accessibility of dye-doped liposomes towards gold nanoparticles for fluorescence sensing of lipopolysaccharide

Author affiliations

Abstract

Inspired by the primitive role of lipopolysaccharide (LPS) and taking advantage of the membrane-philic properties of amphiphilic gold nanoparticles (AuNPs), we established a facile and efficient fluorescence turn-on detection strategy for LPS. Upon binding onto the surface of liposomes, LPS can tailor the accessibility of liposomes towards AuNPs, reminiscent of its primitive function on the surface of bacteria. Thus, while the fluorescence of the dyes labeled on liposomes can be markedly quenched by the membrane-philic AuNPs, the quenching effect can be efficiently prevented by the surface-bound LPS. The de-quenching effect is highly selective to LPS, relative to other negatively charged bio-analytes, which is due to not only the extremely high affinity of LPS to lipid bilayers, but also the unique molecular structure of LPS. Furthermore, this easy-to-construct method offers a limit of detection of ∼0.65 nM, which is comparable to that obtained from the superb synthetic sensors for LPS reported in the literature. This study would open up a new route for the design of sensing systems for LPS exploiting its unique structural pattern and primitive function.

Graphical abstract: Tunable accessibility of dye-doped liposomes towards gold nanoparticles for fluorescence sensing of lipopolysaccharide

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Jan 2017, accepted on 18 Feb 2017 and first published on 21 Feb 2017


Article type: Paper
DOI: 10.1039/C7AN00019G
Citation: Analyst, 2017,142, 1084-1090
  •   Request permissions

    Tunable accessibility of dye-doped liposomes towards gold nanoparticles for fluorescence sensing of lipopolysaccharide

    J. Gao, Z. Li, O. Zhang, C. Wu and Y. Zhao, Analyst, 2017, 142, 1084
    DOI: 10.1039/C7AN00019G

Search articles by author

Spotlight

Advertisements