Issue 4, 2017

Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor

Abstract

Herein a convenient strategy was developed to quantify protein SUMOylation. The amount of target proteins and SUMO proteins conjugated to them could be quantified through luciferase-based bioluminescence detection and the relative bioluminescence was used to evaluate the SUMOylation degree of the target proteins.

Graphical abstract: Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor

Supplementary files

Article information

Article type
Communication
Submitted
18 Nov 2016
Accepted
16 Jan 2017
First published
16 Jan 2017

Analyst, 2017,142, 608-612

Fast and sensitive quantification of protein SUMOylation using a dual luciferase biosensor

Y. Zhang, X. Ke, Y. Liu, Z. Zheng, J. Meng and H. Wang, Analyst, 2017, 142, 608 DOI: 10.1039/C6AN02479C

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